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Gene : |
PECAM1 |
Gene ID : |
5175 |
Uniprot ID : |
P16284 |
Alternative Name : |
PECAM1 |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
C31.3 + JC/70A |
Isotype : |
Mouse IgG |
Immunogen Information : |
Human recombinant CD31 protein (C31.3) & Membrane prepaRation of a spleen from a patient with hairy cell leukemia (JC/70A) |
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1µg/ml); Immunohistology (Formalin-fixed) (1-2µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.