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|
Gene : |
SPN |
Gene ID : |
6693 |
Uniprot ID : |
P16150 |
Alternative Name : |
SPN, CD43 |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
Bra7G |
Isotype : |
Mouse IgM, kappa |
Immunogen Information : |
Immature pluripotent human leukemia K562 cells |
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V). Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at room temp),