|
|
| Gene : |
KRT10 |
| Gene ID : |
3858 |
| Uniprot ID : |
P13645 |
| Alternative Name : |
KRT10, KPP |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
SPM262 |
| Isotype : |
Mouse IgG2a, kappa |
| Immunogen Information : |
Cytoskeletal prepaRation extracted from human ectocervical epithelium |
This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
