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|
| Gene : |
KRT7 |
| Gene ID : |
3855 |
| Uniprot ID : |
P08729 |
| Alternative Name : |
KRT7, SCL |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
OV-TL12/30 |
| Isotype : |
Mouse IgG1, kappa |
| Immunogen Information : |
OTN 11, ovarian carcinoma cell line |
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Western Blot (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
