|
|
Gene : |
EGFR |
Gene ID : |
1956 |
Uniprot ID : |
P00533 |
Alternative Name : |
EGFR, ERBB, ERBB1, HER1 |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
31G7 |
Isotype : |
Mouse IgG2a, kappa |
Immunogen Information : |
Human EGFR purified from A431 cells |
This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, βcellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Digest formalin-fixed tissues with Protease (Sigma Cat. #P-6911) at 1mg/ml PBS, pH 7.4 for 10 min at 37C)
1. Matsumoto Y, Kobayashi M, Shingu K, et al. An anaplastic pleomorphic xanthoastrocytoma with periventricular extension: An autopsy case report and review of the literature [published online ahead of print, 2020 Jun 23]. Neuropathology. 2020;10.1111/neup.12666. doi:10.1111/neup.12666