|
|
| Gene : |
FBXO5 |
| Gene ID : |
26271 |
| Uniprot ID : |
Q9UKT4 |
| Alternative Name : |
FBXO5, EMI1, FBX5 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
EMI1/1176 |
| Isotype : |
Mouse IgG2a, kappa |
| Immunogen Information : |
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein |
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Western Blot (1-2ug/ml for 60 minutes at RT);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
