|
|
| Gene : |
FN1 |
| Gene ID : |
2335 |
| Uniprot ID : |
P02751 |
| Alternative Name : |
FN1, FN |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
SPM539 |
| Isotype : |
Mouse IgG1, kappa |
| Immunogen Information : |
Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose |
Fibronectin is a dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
