|
|
| Gene : |
PMEL |
| Gene ID : |
6490 |
| Uniprot ID : |
P40967 |
| Alternative Name : |
PMEL, D12S53E, PMEL17, SILV |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
HMB45 |
| Isotype : |
Mouse IgG1, kappa |
| Immunogen Information : |
Extract of pigmented melanoma metastases from lymph nodes |
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (2-4ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
