|
|
| Gene : |
HSPB1 |
| Gene ID : |
3315 |
| Uniprot ID : |
P04792 |
| Alternative Name : |
HSPB1, HSP27, HSP28 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
SPM252 |
| Isotype : |
Mouse IgG1, kappa |
| Immunogen Information : |
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells. |
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced ?p29? and ?24K? protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),
