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|
Gene : |
MUC1 |
Gene ID : |
4582 |
Uniprot ID : |
P15941 |
Alternative Name : |
MUC1, PUM |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
E29 |
Isotype : |
Mouse IgG2a, lambda |
Immunogen Information : |
Delipidated extract of human milk fat globule membranes |
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);