|
|
| Gene : |
MUC1 |
| Gene ID : |
4582 |
| Uniprot ID : |
P15941 |
| Alternative Name : |
MUC1, PUM |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
HMPV |
| Isotype : |
Mouse IgG1, kappa |
| Immunogen Information : |
Human breast cancer cell line ZR-75 cells |
This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This MAb reacts with both normal and malignant epithelia of various tissues including breast and colon.
Western Blot (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistology (Formalin-paraffin) (0.5-1.0ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),
