|
|
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
SPM250 |
| Isotype : |
Mouse IgG1 |
| Immunogen Information : |
Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes. |
Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. MAb BM-2 reacts with early precursor and mature forms of human myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (1-2µg/ml); Immunohistology (Formalin-fixed) (0.5-1µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
