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|
Gene : |
TGFA |
Gene ID : |
7039 |
Uniprot ID : |
P01135 |
Alternative Name : |
TGFA |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
MF9 |
Isotype : |
Mouse IgG1, kappa |
Immunogen Information : |
A 17-amino acid synthetic peptide from the carboxyl-terminus of Rat TGF Alpha. |
This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF? ? (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);