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|
| Gene : |
TYMS |
| Gene ID : |
7298 |
| Uniprot ID : |
P04818 |
| Alternative Name : |
TYMS, TS, OK/SW-cl.29 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
TS106 + TMS715 |
| Isotype : |
Mouse IgG1, kappa + Mouse IgG1, kappa |
| Immunogen Information : |
Recombinant human thymidylate synthase (TS106 & TMS715) |
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
