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|
| Gene : |
NANOG |
| Gene ID : |
79923 |
| Uniprot ID : |
Q9H9S0 |
| Alternative Name : |
NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog. |
| Format : |
Purified |
| Amount : |
20 µg |
| Clone name : |
P5A10AT. |
| Isotype : |
Mouse IgG2a heavy chain and ? light chain. |
| Immunogen Information : |
Anti-human NANOG mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NANOG amino acids 1-154 purified from E. coli. |
NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
NANOG antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500~1:1,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:50.
