|
|
| Gene : |
PCNA |
| Gene ID : |
5111 |
| Uniprot ID : |
P12004 |
| Alternative Name : |
Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367. |
| Format : |
Purified |
| Amount : |
20 µg |
| Clone name : |
Pk1A3AT. |
| Isotype : |
Mouse IgG3 heavy chain and ? light chain. |
| Immunogen Information : |
Anti-human PCNA mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PCNA amino acids 1-261 purified from E. coli. |
PCNA is located in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps elevate the processivity of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and takes part in the RAD6-dependent DNA repair pathway. 2 transcript variants encoding the same protein have been found for PCNA gene. Pseudogenes of this PCNA gene have been described on chromosome 4 and on the X chromosome. PCNA is expressed during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA is induced by UV irradiation.
PCNA antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.
