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|
| Gene : |
PKLR |
| Gene ID : |
5313 |
| Uniprot ID : |
P30613 |
| Alternative Name : |
PK1, PKL, RPK, pyruvate kinase isozyme R/L, Red cell/liver pyruvate kinase, PKRL. |
| Format : |
Purified |
| Amount : |
20 µg |
| Clone name : |
PPKLRAT. |
| Isotype : |
Mouse IgG1 heavy chain and kappa light chain. |
| Immunogen Information : |
Anti-human PKLR mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PKLR amino acids 47-574 purified from E. coli. |
PKLR is a pyruvate kinase which catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP. That is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.
PKLR antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500.
