oLeptin, MTS Recombinant Protein

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Product code: 32-1558

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2000 throughout India Order now and get it on next 3-4 Weeks (Major Cities)

Download TDS / Manual MSDS

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100 µg

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Shipping Info:

2000 throughout India Order now and get it on next 3-4 Weeks (Major Cities)

Download TDS / Manual MSDS


Alternative Name :	OB Protein, Obesity Protein, OBS, Obesity factor.
Amount :	100 µg

Source : Escherichia Coli. Leptin Ovine MTS tagged Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17.5 kDa.The Leptin is purified by proprietary chromatographic techniques. The membrane translocating sequence Tag is composed of 10 amino acids Val-Leu-Leu-Pro-Val-Leu-Leu-Ala-Ala-Pro located at the N-terminus. A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

Purification :	Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
Content :	The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Storage condition :	Lyophilized Leptin Ovine MTS tagged although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
AA sequence :	The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.

It is recommended to reconstitute the lyophilized Leptin in sterile 0.02% NaHCO3 not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.