|
|
| Alternative Name : |
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1. |
| Amount : |
10 µg |
Source : HEK.
PDGF-AA Human Recombinant produced in HEK cells is a glycosylated homodimer, having a molecular weight range of 35-45kDa due to glycosylation.The PDGF-AA is purified by proprietary chromatographic techniques.
PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
It is recommended to reconstitute the lyophilized PDGF-AA in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The specific activity was determined by the dose-dependent stimulation of the proliferation of 3T3 cells and is typically 200-1000ng/ml.
