SARS-CoV-2 Nucleocapsid Protein His Tag

Figure-1: ELISA detection of Anti-SARS-CoV-2 Nucleocapsid antibody (ABMX-004) cat. no. 10-2007. An ELISA plate coated with 100 ng of Nucleocapsid protein (Abeomics, Cat. No. 21-1003) was incubated over night at 4C. Different concentrations of Anti-Nucleocapsid recombinant human antibody (ABMX-004) was added to the plate. Goat anti-human Fc HRP was used as detection antibody.

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Figure-1: ELISA detection of Anti-SARS-CoV-2 Nucleocapsid antibody (ABMX-004) cat. no. 10-2007. An ELISA plate coated with 100 ng of Nucleocapsid protein (Abeomics, Cat. No. 21-1003) was incubated over night at 4C. Different concentrations of Anti-Nucleocapsid recombinant human antibody (ABMX-004) was added to the plate. Goat anti-human Fc HRP was used as detection antibody.

Figure-2: Wells of a 96-microtiter plate were coated with 4µg/ml of Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein His Tag. The binding was detected by addition of different dilution of Coronavirus (COVID-19) Nucleocapsid Antibody (Abeomics Cat. No. 11-2003). The reactivity was detected by a HRP-conjugated goat-anti-rabbit polyclonal antibody.

Figure-3: SDS-PAGE analysis of purified Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein His Tag. 2 µg protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.

Figure-4: The checkerboard Sandwich ELISA was carried out by coating the 96 well plate with 200 ng of ABM4H11.1C12 (Cat#10-10035) monoclonal antibody. The nucleocapsid protein was serially diluted from 10 ng to 0.0004ng in triplicates across rows. The 11-2004 detection antibody was applied in serial dilution starting with 200ng/ well across column

Product code: 21-1003

Manufactured By :

IMGENEX India Pvt. Ltd.

Shipping Info:

2000 (throughout India)
Order now and get it on next 3-4 working days* (Major Cities)

Download TDS / Manual MSDS

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Shipping Info:

2000 (throughout India)

Order now and get it on next 3-4 working days* (Major Cities)

Download TDS / Manual MSDS


Gene :	N
Uniprot ID :	P0DTC9
Alternative Name :	Nucleocapsid protein;NP;Nucleoprotein;cov np;Protein N
Amount :	100 μg

Source: E. coli. Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser2-Ala419) of SARS-CoV-2/COVID-19 Nucleocapsid fused with an 6×His tag in N-Terminal.

Purification :	>95% by SDS-PAGE.
Content :	0.5 mg/ml in 50 mM Tris, pH-7.4, 300 mM NaCl and 10% Glycerol
Storage condition :	Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein His Tag is shipped on ice packs. Upon arrival, Store at -20°C. Do not freeze-thaw multiple times.
AA sequence :	The target protein is expressed with sequence (Ser2-Ala419) of SARS-CoV-2/COVID-19 Nucleocapsid fused with an 6×His tag in N-Terminal. MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA.