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Alternative Name : |
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1. |
Amount : |
10µg |
Source : Human 293 cells.
TGFB1 Human Recombinant produced in Human 293 cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa.
The TGFB1 is purified by proprietary chromatographic techniques.
Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
It is recommended to reconstitute the lyophilized TGFB1 in sterile 10mM HCL at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells is less than 0.05ng/ml, corresponding to a specific activity of 2x10^7units/mg.