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| Alternative Name : |
EC 3.1.3.1, IAP, AP, ALPI, Intestinal-type alkaline phosphatase, Intestinal alkaline phosphatase. |
| Amount : |
5 mg |
Source : Calf Intestine.
The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
>1000 U/mg (pH 9.6, 25°C and 0.025M glycin, 10% glycerol as buffer.
