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| Alternative Name : |
Dystroglycan, Dystrophin-associated glycoprotein 1, DAG1, A3a, DAG, AGRNR, 156DAG, MDDGC7, MDDGC9. |
| Amount : |
10 µg |
Source : Escherichia Coli.
DAG1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 30-312) containing 293 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 31.87kDa (calculated).
Dystroglycan 1 (DAG1) is a laminin binding component of the dystrophin-glycoprotein complex which provides a connection between the subsarcolemmal cytoskeleton and the extracellular matrix. The N-terminal domain of alpha-dystroglycan is secreted into the cerebrospinal fluid. The effect of DAG1 on the nervous system remains vague. The complete dystroglycan complex is expressed in a various tissues and has a role in processes such as laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. DAG1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic decrease of DAG1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, making muscle fibers more susceptible to necrosis.
It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. DAG1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
