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| Gene ID : |
223590252 |
| Uniprot ID : |
O43474 |
| Amount : |
100 µg |
| Immunogen Information : |
KLF4 (1 a.a. – 513 a.a.) recombinant protein with an N-terminal His-PTD-NLS tag. |
Recombinant, 6xHis tag
Expression system: E. coli
KLF4 belongs to the Kruppel C2H2-type zinc-finger protein family with three C2H2-type zinc finger motifs. KLF4 is a transcription factor which acts as both an activator and repressor. This protein is a critical regulator which controls expression of key transcription factors during embryonic development and also in monocyte differentiation and macrophage polarization. This factor is one of the four original Yamanaka Factors and has been shown to be essential for maintaining pluripotency of embryonic stem cells. Along with SOX2, OCT4 and c-Myc, it can reprogram somatic or differentiated cells into induced pluripotent stem cells (iPSCs).
The recombinant protein is tagged with nuclear localization signal (NLS), a protein translocation domain (PTD, a poly arginine cell-penetrating peptide) and 6xHis at N-terminal region of the protein. The PTD will allow the entry of transcription factors through the plasma membrane and the NLS will allow entry of the proteins in to the nucleus to exert their biological actions.
Cell culture, WB, ELISA, EMSA
